Tryptic Digest of Cytochrome C
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| Column: |
Vydac® Everest® C18, 5µm, 4.6 x 150mm
(Part No. 238EV5415) |
Mobile
Phase: |
A: 0.1% Trifluoroacetic Acid in Water / B: 0.085% Trifluoroacetic Acid in Acetonitrile |
| Gradient: |
(Time,%B),(0,4),(75,40),(85,90),(95,90) |
| Detector: |
UV at 215nm |
| Flow rate: |
1.0mL/min |
Column
Temp: |
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| Comments: |
For a tryptic digest of cytochrome c, an Everest® column offers unique selectivity which allows the best separation of a group of hydrophilic and hydrophobic peptides.
Vydac® Everest® and four commercial 300Å reversed-phase columns, all C18, 5µm, 4.6 x 15 |
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