Tryptic Digest of Bovine Serum Albumin
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| Column: |
Vydac® Everest® C18, 5µm, 4.6 x 150mm
(Part No. 238EV5415) |
Mobile
Phase: |
A: 0.1% Trifluoroacetic Acid in Water / B: 0.085% Trifluoroacetic Acid in Acetonitrile |
| Gradient: |
(Time,%B),(0,4),(5,4),(80,40),(90,90),(100,90) |
| Detector: |
UV at 215nm |
| Flow rate: |
1.0mL/min |
Column
Temp: |
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| Comments: |
A tryptic digest of BSA injected at high load on an Everest® column provided excellent resolution (110 peaks) compared to four commercial columns (15% better than Column D). Peak numbers shown are the average of five separations on each column.
Vydac® Eve |
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