Tryptic Digest of Fetuin (a Glycoprotein)
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| Column: |
Vydac® Everest® C18, 5µm, 4.6 x 150mm
(Part No. 238EV5415) |
Mobile
Phase: |
A: 0.1% Trifluoroacetic Acid in Water / B: 0.085% Trifluoroacetic Acid in Acetonitrile |
| Gradient: |
(Time,%B),(0,4),(5,4),(80,40),(90,90),(100,90) |
| Detector: |
UV at 215nm |
| Flow rate: |
1.0mL/min |
Column
Temp: |
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| Comments: |
A tryptic digest of fetuin injected at high load on an EVEREST column provided higher peak counts compared to four commercial columns. Peak numbers shown are the average of three separations on each column.
Vydac® Everest® and four commercial 300Å reverse |
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