Two-Stage Purification of a Synthetic 135-mer
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| Column: |
Vydac® 214TP C4, 10µm,10 x 250mm
(Part No. 214TP1010) |
Mobile
Phase: |
A : 0.1M Triethylammonium Acetate, pH 7.0 / B: Acetonitrile |
| Gradient: |
Crude: (Time,%B),(5,0),(40,60)
Deprotected: (Time,%B),(5,0),(25,20) |
| Detector: |
UV at 260nm |
| Flow rate: |
5mL/min |
Column
Temp: |
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| Comments: |
Although generally recommended for oligonucleotides up to 75 bases, Vydac 214TP columns have been used to purify much longer synthetic oligonucleotides. Here a 135-mer is purified by two stages of chromatography on a 214TP column – the first with the 5’-d |
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