Separation of Tryptic Digest Fragments
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| Column: |
Vydac® 218TP C18, 5µm, 4.6 x 250mm
(Part No. 218TP54) |
Mobile
Phase: |
A: 0.1% Trifluoroacetic Acid / B: Acetonitrile |
| Gradient: |
(Time,%B),(0,5),(60,70) |
| Detector: |
UV |
| Flow rate: |
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Column
Temp: |
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| Comments: |
Reversed-phase HPLC is widely used for the separation of peptides in enzymatic digests. In this example, peptides from a tryptic digest of bovine somatotropin (BST) were separated on a 218TP54 C18 column. A fragment from normal BST with asparagine at posi |
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