Fast C18 Reversed-Phase HPLC of Peptides
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| Column: |
Vydac® 218TP C18, 5µm, 4.6 x 50mm
(Part No. 218TP5405) |
Mobile
Phase: |
A: 0.1% Trifluoroacetic Acid in Water / B: 0.1% Trifluoroacetic Acid in Acetonitrile |
| Gradient: |
(Time,%B),(0,15),(4,40) |
| Detector: |
UV |
| Flow rate: |
mL/min |
Column
Temp: |
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| Comments: |
Short columns with low back pressures and low delay volumes can be used for rapid peptide separations with high resolution.
a) 4mL b) 7mL |
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