| Column: |
Vydac® 218TP C18, 5µm, 3.2 x 250mm
(Part No. 218TP53) |
Mobile
Phase: |
A: Methanol:Acetonitrile:Water (20:20:60)
B: Methanol:Acetonitrile:Water (25:25:50) |
| Gradient: |
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| Detector: |
UV at 254nm |
| Flow rate: |
0.5mL/min |
Column
Temp: |
|
| Comments: |
USP requires R not less than 2.0. Hydrocortisone was run under two solvent conditions. Chromatogram B provides a faster analysis and higher sensitivity, while still meeting system suitability requirements. Although not specified for suitability, norethind |
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