| Column: |
Vydac® 218TP C18, 5µm, 3.2 x 250mm
(Part No. 218TP53) |
Mobile
Phase: |
TEA buffer = 4mL of Triethylamine in 2000mL Water, adjusted to pH 3.2 with Phosphoric Acid |
| Gradient: |
Hold 92:7:1 TEA buffer:Acetonitrile:THF for 12 minutes, then ramp linearly to 70:29:1 over 8 min |
| Detector: |
UV at 280nm |
| Flow rate: |
1.0mL/min |
Column
Temp: |
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| Comments: |
USP requires R not less than 3.0. The high resolution of the 218TP column splits resolution peak 1 into two peaks. The second of these, at 4.7 minutes and closest to the vancomycin peak, was used to calculate the R value. |
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