Fast Analysis of Proteins
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| Column: |
Vydac® 238TP3410 C18, 3µm, 4.6 x 100mm,
Vydac® 238TP3405 (identical except 4.6 x 50mm)
(Part No. 238TP3410, 238TP3405) |
Mobile
Phase: |
A: 20% Acetonitrile, 0.1% Trifluoroacetic Acid / B: 45% Acetonitrile, 0.1% Trifluoroacetic Acid |
| Gradient: |
(Time,%B),(0,0),(4,100) |
| Detector: |
UV at 215nm |
| Flow rate: |
as noted |
Column
Temp: |
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| Comments: |
In these two chromatograms, the higher flow rate possible on a 50-mm-long column produces a shallower composition gradient on a per-volume basis and provides slightly higher resolution than a 100-mm-long column. Separation times are similar. |
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